Search results for "Bradyrhizobium japonicum"
showing 5 items of 5 documents
Changes in the physiological and agricultural characteristics of peat-based Bradyrhizobium japonicum inoculants after long-term storage
2000
International audience; Commercial soybean inoculants processed with sterilised peat and stored at 20 °C for 1–8 years were used as experimental materials to assess the changes in the physiological activity of Bradyrhizobium japonicum after storage. Viable counts decreased and physiological characteristics of the bacterium changed during storage, with an increase in the time taken for colony appearance on a medium without yeast extract, an increase in the lag time for nodule appearance on soybean grown in glass tubes and a decrease in survival on seeds. All the inoculants produced a significant increase in grain yield in a field experiment. The percentage of efficient cells in the field (re…
Effect of carbon source supply and its location on competition between inoculated and established bacterial strains in sterile soil microcosm
1999
The aim of this work was to study how the location of a carbon source and of bacterial cells in soil can enhance the growth of a bacterial inoculum. Studies were performed using irradiated soil and two pairs of strains of Bradyrhizobium japonicum and Pseudomonas fluorescens. For each species, an antibiotic-resistant mutant was used as inoculant and introduced into a sterile soil pre-colonised with its parent strain. The inocula and a glycerol amendment were introduced together or separately into soil using porous microgranules or by spraying a suspension onto the bulk soil. Competition was assessed by plate counts of both inoculant and established populations. Both B. japonicum and P. fluor…
Novel avidin-like protein from a root nodule symbiotic bacterium, Bradyrhizobium japonicum.
2005
Bradyrhizobium japonicum is an important nitrogenfixing symbiotic bacterium, which can form root nodules on soybeans. These bacteria have a gene encoding a putative avidin- and streptavidin-like protein, which bears an amino acid sequence identity of only about 30% over the core regions with both of them. We produced this protein in Escherichia coli both as the full-length wild type and as a C-terminally truncated core form and showed that it is indeed a high affinity biotin-binding protein that resembles (strept)avidin structurally and functionally. Because of the considerable dissimilarity in the amino acid sequence, however, it is immunologically very different, and polyclonal rabbit and…
Legume inoculants to reduce soil emissions of nitrous oxide
2012
Nitrous oxide (N2O) is a potent greenhouse gas involved in depletion of the ozone layer and mainly arising from the soil and from agricultural activities. Within this framework, INRA teams inoculated soybean plants with natural strains of Rhizobia bacteria. Convincing results regarding the consumption of N2O greenhouse gases were obtained.
Caractérisation génotypique et le phénotypique de souches de Rhizobia par rapport à leur capacité à réduire le protoxyde d’azote (N2O), puissant gaz …
2012
Rapport de Stage de Master 2 EA MERS CT3; Master